Saturday, August 31, 2019

Of all the characters in the Crucible Essay

Of all the characters in the Crucible, John Proctor is perhaps the most admirable. He has strong personal principles and the courage to stand by them. Proctor strives to do what is right. He tries very hard to please Elizabeth because his affair with Abigail Williams has left him with a guilty conscience. In Act 2, he compliments Elizabeth’s rabbit stew, even though much of it is his own work. † It’s well seasoned, † he says. He labors constantly, for her well being, believing that this will make their relationship successful. Proctors first appearance, in Act 1, is his arrival from chopping wood in the forest. During much of Act 1 he is working. For example, he returns from a hunting trip and from planting seeds in his garden, on a cold winters day. Proctor believes in discipline and can be very strict if he needs to. As is illustrated in Act 2. Mary Warren disobeys him and goes to the Salem trials as an official. On her return, he scolds her shouting, â€Å"I’ll official you†¦ I’ll whip the devil out of you! † He is against the trials, perhaps because he was never a strong Christian. In Act 2 he is told to recite the Ten Commandments. He starts, â€Å"Thou shalt not steal†¦covet thy neighbours goods†¦ make unto thee any graven image†¦ bear false witness†¦ make unto thee any graven image. † finally stuttering and failing. We get an insight to his values in Act 2, when he shows his disapproval of the Salem Witch trials, saying: â€Å"It’s a strange work†¦ to hang old women†. This is further illustrated, later in the same act, when his wife, Elizabeth, learns that she is accused of witchcraft. Proctor, deeply shocked, becomes very protective towards her. He says that they shall resist the charges and that â€Å"There will be no noose†¦fear nothing†. Johns courage to stick by his beliefs is illustrated at the end of Act 2, when he stands up to court officials in a struggle to oppose the charges against his wife. He tears up the arrest warrant, throws out the officials and rages â€Å"Damn the Deputy Governor! Out of my house! † At the end of the play, in Act 4, John faces the ultimate test of his courage and moral values when he is faced with signing the document, where to lie would save his life. Proctor cannot sign the lie and exclaims: â€Å"Because it is my name! †¦ Because I lie and sign myself to lies! † Abigail Williams is undoubtedly the most despicable character in the play. Her young and innocent appearance conceals a sly, selfish and callous girl. Abigail had once been John Proctor’s lover, but was then rejected. She cannot bear the fact that John no longer loves her the way that she believes he used to, and that he wants to pretend that nothing ever happened. In Act 1, John says to her: â€Å"Wipe it out of mind. We never touched. † Abigail is a deceitful person who does not care about the outcome of her actions. Her envy leads her to make false accusations against Elizabeth Proctor. She is a skillful liar. This is illustrated in Act 3 when Abigail, after purposely wounding herself, argues, in court, against Elizabeth saying: â€Å"I have been hurt Mr Danforth†¦ I done my duty pointing out the devil’s people – and this is my reward? To be mistrusted, denied, questioned†¦? Abigail has a bad name in the village because she is often seen doing mysterious things, such as conjuring spirits and more generally because of her malevolent character. This is illustrated in Act 1 where Betty exclaims, â€Å"you did, you did! You drank a charm to kill John Proctors wife! † Abigail then smashes Betty across the face and shouts â€Å"shut it, shut it! † As the thoughts and ideas of witchcraft and devil worship spread through Salem, Abigail finds an easy way to get rid of her enemies without degrading her name any further. By accusing them, or convincing others to accuse, Abby is able to place many innocent people on trial for witchcraft. An example of this occurs at the end of Act 3, when Abigail, having convinced an assembly of girls to help her accuse Mary Warren, manipulates the situation. The assembly slowly descends into a screaming crisis, which makes Mary sound like a mad woman. Abigail uses this technique many times in the play. On this occasion, Mary Warren blames Proctor to save her life. The underlying evil of mindless persecution pervades this play. There are many characters to admire and despise. Abigail Williams deserves some sympathy, as she was callously cast aside by Proctor. Nevertheless, her decent into evil, surpasses them all Show preview only The above preview is unformatted text This student written piece of work is one of many that can be found in our GCSE Arthur Miller section.

Friday, August 30, 2019

Effect of pH on Invertase Activity

ABSTRACTInvertase is a type of enzyme, a natural catalytic agent for biochemical reactions, can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor the enzymatic activity of invertase. Invertase was subjected to different pH (3.87, 4.0, 5.5, 7.3 and 10.55) of buffer solution and was observed under 540 nm absorbance using spectrophotometer. After observation and analysis, a peak (optimum pH) was observed by plotting absorbance versus pH.INTRODUCTIONEnzymes are proteinaceous catalysts, which speed up the rate of a biochemical reaction. They reduce the activation energy that is essential for starting any type of chemical reaction. With a low energy requirement for activation, the reaction takes place faster. The overall performance of an enzyme depends on various factors, such as temperature, pH, cofactors, activators and inhibitors. Inver tase is an enzyme which is usually found in plants. It acts as a catalyst for the hydrolysis of sucrose.Sucrose is a disaccharide composed of glucose and fructose linked by a glycosidic bond. When this bond is cleaved in a hydrolysis reaction, an equal amount of glucose and fructose. Invertase is a significant enzyme because glucose is an important product of photosynthesis. Invertase is also used in the confectionery industry where fructose is preferred over sucrose because it is sweeter and does not crystallize easily.Enzymes are affected by changes in pH. Extreme pH values generally result in loss of activity for most enzymes. Furthermore, there is a most favorable pH for enzyme – the point where the enzyme is most active. This point is known as the optimal pH. The aim of this experiment is to find out the range of pH which invertase is effective. The objectives of this experiment  are: to extract invertase from Baker’s Yeast and to determine the effects of change s in pH on reaction rates of an enzyme-catalyzed reaction.MATERIALSThe materials used in this experiment are: Baker’s Yeast, Sucrose Standard Solution (100 mg/L), Concentrated HCl, 0.5 M KOH, DNS reagent, 0.1 M buffer solutions (pH 1, 3, 5, 7, 9, 11), ucrose solution (10 g/L), test tubes, pipets, beakers, volumetric flasks, paraffin film, hot plate and UV-Vis Spectrophotometer.METHODOLOGYExtraction of invertase from yeastTo extract the invertase from Baker’s Yeast, 0.25 g of it was dissolved in distilled water to make a 250-mL solution. When the solution is prepared (complete dissolvation of Baker’s Yeast) it is then allowed to stand for 20 minutes at room temperature. Provided that the sediments form, the supernatant must be collected as it will be used as the enzyme stock solution that will be used in the succeeding experiment. Sucrose Assay Using Dinitrosalicylic Colorimetric MethodIn preparation of this part of the experiment, a series of test tubes were pre pared as follows: Tube No. Blank 1 2 3 4 5 6 mL sucrose std. solution 0 0.25 0.50 0.75 1.00 1.25 1.50 mL distilled water 1.50 1.50 1.25 1.00 0.75 0.50 0.25After, 3 drops of concentrated HCl (0.05mL) were introduced to each test tube. Noted that the tubes were mixed well and then incubated after at a 90 degrees Celsius water bath for 5 minutes. After the incubation, 0.15 mL of 0.5 M KOH was added to neutralize the solution. Another 2.80 mL of 0.1 M buffer solution at pH 5 were added, then the solution was mixed well again. Then, 3 mL of DNS reagent was added before the test tubes were immersed in a water bath at 95 degree Celsius for 10 minutes to develop the characteristics of a red-brown colour solution. After cooling, the solution were subjected into spectrophotometry to measure the absorbance at 540 nm. Effect of pH on Invertase ActivityIn finding the effect of pH on invertase activity, six numbered test tubes were prepared with 2.90 mL appropriate 0.1 M buffer solution as shown below: Tube No. 1 2 3 4 5 6 pH buffer solution 0.1 0.3 0.5 1.7 1.9 1.11Then, 0.10 mL enzyme stock solution was added to each test tube. After mixing thoroughly, all test tubes were incubated in 60 degrees Celsius water bath for 5 minutes. When the time was right, another 1.50 mL of sucrose was added. The solution was then incubated again and treated to the same water bath for the same amount of time, 5 minutes. Then, 3 mL of DNS reagent was added before immersing the solution in a water bath (95 degrees Celsius) for 10 minutes until the solution turns into a red-brown colour solution. After cooling the first test tube, blank solutions were prepared by following steps 1-4 again, but instead of using the enzyme stock solution, denatured enzyme was added. All the test tubes containing the solution were then subjected to spectrophotometry to measure the absorbance at 540 nm.EXPERIMENTALSucrose Assay Using Dinitrosalicylic Colorimetric MethodA. Materials used Sucrose Standard Solution, D istilled Water, Concentrated HCl, 0.5 M KOH, 0.1 M Buffer Solution, DNS Reagent, and UV-Vis Spectrophotometry. B. Procedure After collecting the supernatant from the enzyme stock solution, each test tube were introduced to 3 drops of conc. HCl before incubating at 90oC water bath for 5 minutes. 0.5 M KOH was then added to neutralize. Then, 2.80 mL of 0.1 M buffer solution was added before the solution was introduced to DNS reagent. The solution was in water bath at 950C for 10 minutes (until it is a red-brown solution). After cooling, it is subjected to spectrophotometry to measure absorbance at 540 nm. Effect of pH on Invertase ActivityA. Materials usedBuffer Solution, Enzyme Stock Solution, 1.50 Sucrose Solution, 3 mL DNS Reagent, Test Tubes, UV-Vis Spectrophotometry.B. ProcedureAfter preparing the required test tubes, they were introduced with 0.10 mL enzyme stock solution before being incubated for 5 minutes in a water bath at 600C. Then, 1.50 mL sucrose solution was added befor e the solution was incubated again for 5 minutes in a water bath with the same temperature. After cooling, 3 mL DNS reagent was added before immersing the test tubes  again in a water bath at 950C until the red-brown color appears. Repeat steps 1-4 but this time, instead of adding the enzyme stock solution, add the denatured enzyme. After all the test tubes were prepared, they were sunjected to UV-Vis Spectrophotometry to measure absorbance at 540 nm.Image 1. The red-brown coloration after water bathRESULTSSucrose Assay Using Dinitrosalicylic Colorimetric Method The following table shows the results from the UV-Vis Spectrophotometer of Sucrose Assay using DNS Colorimetric Method:Test Tube No. Amount of Acid-Hydrolized Sucrose Absorbance Blank 0.0 0.000 A 1 0.56 0.335 A 2 1.11 -0.456 A 3 1.67 1.248 A 4 2.22 1.800 A 5 2.78 -0.238 A 6 3.33 -0.319 A Table 1. Results of Sucrose Assay using DNS Colorimetric Method The students were also asked to plot the hydrolized-sucrose standard curv e by plotting Absorbance against Concentration (mg/mL)Chart 1. Standard Curve of Absorbance against Concentration.Effect of pH on Invertase Activity The following table shows the results from the UV-Vis Spectrophotometer in respect to the Effect of pH on Invertase Activity:pH Amount of Acid-Hydrolized Sucrose Absorbance Blank 0.0 0.000 A 3.87 2.02 0.162 A 4.0 9.12 0.78 A 5.5 12.6 0.975 A 7.3 1.883 0.151 A 10.55 9.33 0.748 A Table 2. Results of the Effect of pH using Colorimetric Method.

Thursday, August 29, 2019

Internship paper Essay Example | Topics and Well Written Essays - 1250 words

Internship paper - Essay Example For this stage, I have been in communication with the owner, Inka Kardys and a support staff that handles human resources administration (Kardys is also the manager and would be my immediate supervisor throughout my internship). Here, I was asked to provide a lot of personal information as much as I was required to provide my skills and capabilities. There was a lot of focus on the interview and afterwards, it was all formality. I passed and was requested to relocate, then start my training. I started to work at Umbruch in July 1, 2009. I was required to deliver at least 40 hours per week just like any of their ordinary employee. I have to be in the office at 8 in the morning till 5 in the afternoon. I have to work there five days a week. As previously mentioned, I worked for Kardys, the firm’s manager. And so my responsibilities were mainly to assist her as he does her managerial duties as well as other tasks that she would assign me to do. From July until August 16 2009, the last day of my internship, I was subjected to a structured training which covered those duties that are related to all the operations of various departments in the company. For the first half of week, I underwent orientation and familiarization of the workplace. The orientation focused on the nature of the company and how individual employees could contribute in the firms organizational objectives. It was very thorough and by the end of this stage, I have a fairly sufficient knowledge in regard to the company, their products and services, how they work and the organizations’ goals and objectives. During this stage, I noticed that the process seem to want to inculcate in the employee the corporate culture and organizational values which I found to be important in how I dispensed with my duties and responsibilities as an employee. It was all very clear

Wednesday, August 28, 2019

Discussion questions1-3 Essay Example | Topics and Well Written Essays - 250 words

Discussion questions1-3 - Essay Example The interpreter does not differentiate between exception and syntax error, it continues to interpret and execute the source code till possible. However, it stops interpreting where finds a syntax error with an error message. In this regard, it is achievable for interpreters to handle the syntax errors as exceptions to continue an execution of a source code/program even after the syntax error. Defensive programming is to investing more time in writing a secure program by validation of extensive of input, avoiding garbage values and standardization of exception handling. The defensive programming relates to variables as in the defensive programming we need to use naming scheme (preferably Hungarian Variables) begins with int, str and providing meaningful variables names. All the variables should be explicitly initialized to avoid garbage values, long script should be break into sections that facilitates reusability and makes easy debugging. Hence, it can be said that the defensive programming avoids not only syntax errors but also some semantic errors while using the above methods, so that when viewing variables the programmer explicitly knows for what purpose the variable is declared and used in a program. Spreadsheet model is one of the major business models that is ubiquitous and allow us to develop more complex and detailed models than traditional mathematics allows. It facilitates business communication especially performing modeling tasks and accounting statements in effective and efficient manner, as it is more-powerful and refined tool. This is the reasons for which spreadsheet modeling is high in demand of recruiters. Modeling helps us to make informed decisions in any business. We can model a system before its actual implementation to know any defect in the model, test the model and can identify pros and cons of the modeled system. Modeling can be used to investigate alternate ways of doing business, without investing in a

Tuesday, August 27, 2019

Utilitarianism Essay Example | Topics and Well Written Essays - 1250 words - 1

Utilitarianism - Essay Example The paper will also give some examples to illustrate the situation. As mentioned above, maximum happiness and minimum suffering are the two central concepts of the Utilitarian approach. In other words, the Utilitarian view supports any course of action that would maximize the over wellbeing of the society, or the greatest number of people. According this philosophical theory, happiness of people has to be sacrificed only if such an act would bring greater happiness to more number of people. Stuart Mill, one of the major contributors to the theory of Utilitarianism, gives a clear-cut definition of Utilitarianism. According to him, â€Å"actions are right in proportion as they tend to promote happiness, wrong as they tend to produce the reverse of happiness† (as cited in Bykvist, 2010, p.21). As per the Utilitarian view, an action that would give happiness to greatest number of people is supportable even if it may physically or mentally harm some other people but fewer in numbers. One of the major criticisms against the Utilitarianism is that t his philosophical theory fails to define what will maximize happiness. Opponents claim that the notion of happiness may vary from person to person in accordance with their moral beliefs. In the words of Taranovsky (2003), another central concept of this moral theory is that one person’s happiness should never be more important than that of others and there is no good reason to follow a rule of conduct which is detrimental to the happiness of the majority of people. Under this concept, many fundamental laws of conduct and practices of justice are not supportable as they are not likely to make greatest number of people happy. Often, the most criticized aspect of Utilitarianism is that this ethical philosophy supports homicide under certain circumstances. According to Utilitarian approach, even homicide is justifiable if it contributes to the happiness

Monday, August 26, 2019

Yahoo Inc Case Study Example | Topics and Well Written Essays - 1750 words

Yahoo Inc - Case Study Example It is a multilingual consumer interface accessible in 20 foreign languages over the planet, including English. Jerry Yang along with David Filo who were scholars from Stanford created the website in the first month of 1994. In the beginning, the site was named after Jerry referring to it as a guide to World Wide Web (WWW). It served the purpose of a directory since it linked with other websites in a chronological order. It was again named â€Å"Yahoo!† three months later in the same year, where it gained remarkable viewer hits. The exclamation mark was added as a trademark to register it under the law to avoid the resource infringement. In an effort to improve the service range to motivate the users to utilize extra time at the site, Yahoo launched an online e-mail service called four one one (411). The firm acquired Rocket mail, in addition to, Classic Games lines to expand its varieties that were by now common. In the year 2000, on the onset of millennium, Yahoo recorded the ir best results in the dot-com period when the revenue collection rose by 4.5%. In addition to that, the company merged with Google command searches since one would access the sites on either accounts. This resulted to Yahoo inventing a modern technology that would enable its viewers to enjoy their services directly. The deal that existed between the two firms was broken when the invention took place-precipitating creation of 2GB storage accounts, the opposite of Google’s 1GB. Major Issues or Problems of the Organization Every organization must face challenges in the formative stages and during the routine operations. Yahoo Corporation was faced with the challenges of account hackers, brain drain and cyber crimes. The common and most prevalent crisis at Yahoo is the hacking of accounts where respondents claim that at one time or the other their accounts have been hacked. Hacking is an art where an individual intentionally or unintentionally access an account of the other for security reasons or by clicking malicious links. Account hacks causes are mostly use of general Internet terminals or Wi-Fi prior to the attack. Moreover, they connect to links that might result to malicious locations. Almost every time, the hacked accounts, are used to distribute spams and frauds. The best way to avoid this is by altering the passwords to a secure one or applying different passwords foe every website frequented. Extra vigilance should be practiced when using public terminals. Another challenge is the brain drain experienced at Yahoo Corporation whereby staffs are leaving the firm at an alarming rate to set up competing firms. The best employees at the firm are speedily leaving to join other rival firms or set their personal firms. This is a challenge to Yahoo because these gone staffs understand the weaknesses and strengths of the firm which they can employ to their gain. Lastly, cyber crimes are illegal measures on the net that pose security issues to the users. T he cyber crimes comprise of software piracy, e-mail spying and credit card swindles which invade the privacy of the users. It is feared that the modern fraudsters loot more money through Internets than robberies. These crimes are directed to individuals, properties or institutions. Cyber crime rules have been enacted to curb the spread of these crimes to innocent, unsuspecting users (Wall, 2007). Competitive Environment As borrowed from Yahoo 10K, Yahoo chiefly challenges other businesses to draw users to its website and promoters to its marketing services. Yahoo expects the market to develop progressively more competitive in the event that online marketing persists to nurture and gain reception on an international basis. Yahoo’

Sunday, August 25, 2019

Why it is necessary for HR practitioners to monitor the external macro Essay

Why it is necessary for HR practitioners to monitor the external macro environment - Essay Example The business environment is made up of many such factors that can affect the business in the shot and long term both. Therefore it is important to know and study about these factors and evaluate the changes and their impact on the organization. The external environment is made up of the all the forces present outside the organization that affects or has the ability to affect business’s ability to achieve its objectives. The external business environment is broadly categorized as the micro and macro external environment, the paper focuses on the external macro environment therefore we will discuss about that in detail. External Macro Environment: The external macro environment is made up of factors which are beyond the control of the organization. These include the PESTEL (political, economic, social, environmental and legal) factors. These factors are interlinked and cannot be distinct from each other easily. The effects these factors have on the businesses vary business to bu siness and from time to time. The effect of changes in the macro environment over the business is indirect, that is it affects the business through some changes. However the change in business doesn’t affect the environment in return. ... However the changes in the environment that affects the business occur constantly and they are difficult to predict. (BATES. 2005; KROON. 1995) When we speak about environment and its relationship with human resource management it refers to the combination of factors that influence the working and execution of the HR department. Any function of the organization, as mentioned earlier, cannot work in isolation and are interdependent on a lot of factors internally within the organization and outside the organization too, therefore for smooth operations and decision making it is essential to take into account of these factors which can influence HR polices, procedures and practices. The best framework to study the external macro environment, its components and how they affect the functioning of HR department is by the PESTEL analysis. I will further discuss the possible factors that lie in the PESTEL analysis and influences the HR function. Economic Factors: Economic environment is compo sed of many economic factors like populations, fluctuation in economic and trade cycle, national income, labour market conditions, interest rates and globalization of economy. This is the most important of all the factors prevailing in the environment that affects an organization the most including the HRM function. This could be made evident by the scenario for e.g. when company’s performance in well due to the boom in the trade cycle, it will attract more business and due to increase in business it may need to hire more labour from the market and it might face difficulty in hiring the required labour from the market. To serve the purpose the organization may be willing to pay extra compensations

Abrasive Consultant Essay Example | Topics and Well Written Essays - 1000 words

Abrasive Consultant - Essay Example Peter Drucker was of the opinion that â€Å"We know nothing about motivation. All we can do is write books about it.† (Spillane and Martin, 2005, pg. 46). Although Drucker had a rather hopeless view on the subject, from a psychological perspective it is possible to conjecture about an individual’s agendas. To consider the possible reasons for Julia’s behavior, we must first analyze her personality type. An abrasive personality is one that annoys others and causes hostility as a result. The dictionary meaning also lists synonyms that include ‘overly aggressive’. Abrasive behavior that is erratic, resulting in heightened conflict, the blame game, ambiguity, overt contempt, and defensiveness is typically seen as an outward symptom of hidden feelings of inadequacy and fear. In addition, there is a self-generated environment which, according to theorists, is another complex layer in the relationship between human behavior and environment. The concept post ulates a particular type of behavior produces conditions that affect future behavior. For instance, individuals who have abrasive personalities, contribute to the creation of a social environment that is hostile, leading to further aggression from them. This is a self-perpetuating cycle sustained by the aggressive individual. Granted the state of a parent’s health can be an emotional issue, but Julia could easily have made a few calls herself to verify her mother’s health. This lapse and her subsequent rant at Cathy at the office indicate a tendency toward manipulative behavior, either conscious or subconscious. Cathy’s feelings of guilt are a direct result of her being emotionally blackmailed by Julia into feeling guilty. This manipulation results in a role reversal, where Julia, who in actuality has a subordinate position, assumes a dominant role, forcing Cathy into a subservient one. Indeed, Julia may derive a secret pleasure from this short-lived but repeate d forced reversal and dominance. It also results in the vital issue of client dissatisfaction not being addressed, again, due to the manipulative tactics Julia may have adopted to avoid being warned.

Saturday, August 24, 2019

Business law Essay Example | Topics and Well Written Essays - 1500 words - 10

Business law - Essay Example In this regard following issues can be framed: In legal sense Contract is defined as â€Å"an agreement enforceable at Law† where as every promise and every set of promises, forming the consideration for each other, is an agreement. And each promise is a combination of an offer by one person and acceptance of that offer by an other person. 2(The Indian Contract Act, 1872) The basic components of this agreement are: In case of deal with Slump Ltd through Mr. Boom offer was made by Mr. Boom to purchase the cars for use on basis of advice rendered by Mr. Smooth; a salesperson employed by Big Deal Ltd. Mr. Smooth accepted the offer by acting in accordance with the offer for price tag which forms the consideration of 20,000/- each. Similar is the case of deal with Ms. Market. Hence, in both case there were legal contracts between the parties on the ground that all the three components i.e. offer, acceptance and lawful consideration were present. A contract of sale of goods is a specific type of contract. I contract maybe a legal one but still not constitute a sale of goods contract. In order to do so a contract should correspond to Sec: 2(1) of the Sale of Goods Act 19793, which provides that, â€Å"A contract whereby the seller transfers or agree to transfer ownership in goods to the buyer in return for a money consideration called the price.† Both the deals under discussion in the current debate qualify to be a contract of sales of goods where ownerships was transferred to buyers for a price tog of 20,000/- and 5,000/- in case of slump Ltd and Mr. Market respectively. Cars purchased were legal consideration in this regard. Certain professions carry certain implied conditions attached to their products and services. However, law describes the implied conditions in general too. Section 14 of Sales of Goods Act, 1971, speaks about these implied conditions. This section defines that goods to be sold shall be of